I want to thank all of those who responded to me regarding their experiences
with DAKO antibodies and their new Medimachine. It was requested that I
summarize the responses.
I received a total of 8 responses. 6 reported good or comparable results to
other manufacturers with the following comments:
- Antibodies good but not optimized for flow/need tittering.
- DAKO good for providing samples of antibodies for testing
- Isotypes concentrations are not matched to the specific antibody
concentrations so they need adjusting
- Most of the antibodies are IGG1 therefore reducing need for many
different isotypes
- The PE appeared cleaner than other manufacturer's PE conjugated products
- All reports were that DAKO was less expensive
2 reports of questionable results were made. One center appeared to find that
the antibodies performed great on normals but yielded questionable results on
clinical cases so they returned to alternative manufacturers.
As far as the Medimachine was concerned. I received two specific responses that
were very positive. Both felt that the machine made great advances toward a
standardized approach to cell disaggregation. One other comment also praised it
for reducing technologist exposure to infectious samples.
Thanks again for all the comments. We have decided to give some a try on
parallel testing.
Haywood Pyle
King Faisal Specialist Hospital and Research Center
MBC#3, Box 3354, Riyadh 11211, Saudi Arabia
pyle@kfshrc.edu.sa
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