Viki Mosiman Robert H Lurie Cancer Center Flow Cytometry Core Facility Northwestern University Medical School Chicago, IL vlm646@nwu.edu My thanks to everyone who has responded regarding my question about the difficulty of detecting large cell lymphoma(B-cell type) by flow. About half of the respondents replied they were having the same problem(positive nodes by Surg.. path ,negative by flow cytometry). Several suggested also trying cytoplasmic staining for Kappa/Lambda along with cd20 instead of cd19 to detect Bcells( that was also done on our large cell lymphoma cases with no success in detecting clonality). Another third suggested that the cells are lost due to their fragile nature(lost in processing or when run on the instrument due to shear forces),which I believe is a very good possibility. Thanks again for your help! Clinical Flow Lab Northwestern Memorial Hospital Chicago ,Ill.
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