Viki Mosiman Robert H Lurie Cancer Center Flow Cytometry Core Facility Northwestern University Medical School Chicago, IL vlm646@nwu.edu I am interested in an opinion on the best way to detect Large-cell lymphoma(B Cell type) in lymph nodes. We currently disaggregate nodes then surface stain using 45/19/kappa/lambda. Although we often have success ,there are cases where clearly the node is a malignant B-cell lymphoma by morphology in Surgical Pathology but we see no evidence in Flow. We have tried blocking with fetal calf and mouse serum. We have tried different gating strategies such as 45ss combined with 19ss gating or combined with fs. We have tried different staining combinations -19/5/kappa/lambda-45/19/10/kappa&45/19/10/lambda. We have tried live dead assays(7AAD) combined with surface staining in case a node was necrotic. Someone even suggested injecting nodes with PBS to express cells instead of mincing and grinding lymph nodes(they thought this might be a more gentle process). We don't seem to have the same problem with other malignant lymphomas in lymph nodes,just with the diagnosis of Large-cell lymphoma ( B-Cell type). Any suggestions would be greatly appreciated. Thanks for your help! Clinical Flow Lab Northwestern Memorial Hospital
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