Dear Flow Friends, To those who responded to my question, I was hoping to thank you all individually for taking the time to help me. I will still try to do so, but time constraints will not allow me, and it has been far too long to wait any more. Anyway, THANK YOU for your help. I did an experiment to compare NH4CL vs lysing via Gentrak... Gentrak won. It worked quite well also! I have one last question, and it is quite simple. I cannot use my usual resources as I am writing at home, so I will ask here, and in the next message also for maximum visibility (not obvious in the title of this message). Perhaps, if you are still reading this, you can give a quick answer: What is the usual percent positive of CD19 for mouse blood? I believe it is 30-40%, but would like backup. Thanks so much! Your Fellow Friend in Flow Patricia Echeagaray Flow Cytometry Services Southern Research Frederick echeagaray@sri.org
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