cytoplasmic staining

From: P. VAIGOT (vaigot@infobiogen.fr)
Date: Thu Oct 02 1997 - 05:32:29 EST


Dear group

Actually we try to stain dissociated epithelial cells and we have two kinds
of problem.
- First, staining of cells in suspension with antibody anti integrin
subunit seems very difiicult to detect and is not reproductible.

- Second, does anybody have some practice of VITAL staining of cells with
an intracellular cytoplasmic marker such as cytokeratin?

Does anybody have some experiment which would help us?


        Thanks

                                PIERRE



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