Dear group Actually we try to stain dissociated epithelial cells and we have two kinds of problem. - First, staining of cells in suspension with antibody anti integrin subunit seems very difiicult to detect and is not reproductible. - Second, does anybody have some practice of VITAL staining of cells with an intracellular cytoplasmic marker such as cytokeratin? Does anybody have some experiment which would help us? Thanks PIERRE
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