You might try a product called Lympholyte-M by Cedarlane Laboratoy of Hornby, Canada. Phone number (416)878-8891. It's available from Accurate Chemical and Scientific Corp in the US at phone# 1-800-645-6264. I'm not sure who has European distribution for it. It is Nycograde Polysucrose 400 and Sodium Diatrizoate at a density of 1.0875 plus or minus 0.0010 at 25 degrees centigrade. Ph is 6.9 NYCOGRADE is a registered trade mark of Nycomed Pharma AS of Oslo, Norwary. You need a different density of separation media for mouse cells than you do for human cells as the mouse cells are somewhat smaller in size and denser. Lympholyte-M is designed for mouse lymphocytes, I don't know exactly how many monocytes you'll get with it, but it will be better than standard Ficoll-Hypaque, I'm sure. Give Accurate's technical service people a call maybe they can tell you what you can expect to get. Cedarlane also makes a Lympholyte-rat with a differnt density for separating rat lymphocytes cells as well.
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