Re: Clumping of SV40 Transfected Cells

From: Shelton Murinda (sem124@psu.edu)
Date: Tue Sep 30 1997 - 21:24:52 EST


I am experiencing clumping of clumping of SV40 transfected human colon
cells after trypsinization prior to fixing with paraformaldehyde.  This is
affecting apoptosis determinations.  Does anyone have a quick fix?

SHELTON E. MURINDA
____________________________________________________________________________
Food Microbiology Laboratory
120A Borland Building
Department of Food Science
The Pennsylvania State University
University Park, PA 16802
Phone: 814-862-8475 (H); Phone: 814-863-2970 (W)
Fax: 814-863-6132

"The lizard that fell from the high iroko tree praised himself for having
jumped such a great height"
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