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From: Dimitri Scholz (dscholz@kerckhoff.mpg.de)
Date: Mon Sep 29 1997 - 08:46:22 EST

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Hi, flowers!

I am a microscopist and now starting the flow. My first experiments did not
work because I lost a majority of the cells during centrifugation. My
questions are:

1. Is it possible to use the indirect reaction or I should bind the FITC
directly to the primery antibody?
2. How can I optimize the centrifugation: time, g, volume, temperature.
3. Is there any good book about the flow for the beginner?

Thank you for any help. Dimitri

Dr. Dimitri Scholz              |  voice    ++49 6032 705-403
Max-Planck-Institute            |  Fax      ++49 6032 705-419
Dept. of Exp. Cardiology        |  Email    dscholz@kerckhoff.mpg.de
Beneke-Str.2                    |
D-61231 Bad Nauheim             |
Germany                         |

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