The trouble with dot plots, as I understand it, is that once a
particular region is saturated with dots, there is no way one can
intuitively estimate the percentage of cells in that region.
It seems to me (and it is quite likely that my friend and colleague Dr.
Roederer will disagree) that dotplots are OK when the region of interest
is one of extremely few events.
I also prefer dot plots with kinetics, because fluctuations in
collection rate can obscure a response of a small population of cells
that rise above the baseline.
ron
Ronald L. Rabin, M.D.
NIAID/NIH
Building 10/Room 11N228
10 Center Drive MSC 1888
Bethesda, MD 20892-1888
Phone: (301) 402-4910
FAX: (301) 496-7383
Flow-ers,
Mario Roederer said:
> Of course, you will agree that dot plots are completely inappropriate.
> (Everyone: please stop publishing data with single-color dot plots!)
You've lost me on this one. Being a relative new-comer to flow
cytometry, I don't see what is wrong with dot plots. Please educate
me. If dot plots are "completely inappropriate", is there ever a
situation when they would be appropriate to use?
Thanks,
Ken Schafer
kschafer@lucy.tli.org
Kenneth A Schafer
Lovelace Respiratory Research Institute
PO Box 5890
Albuquerque, NM 87185
505-845-1126
505-845-1198 (fax)
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