For Keith Bahjat, trough the cytometry list because my postmaster doesn't like his address. That's for the species cross-reactivity chart: Dako Rabbit anti-human T cell, CD3, code n. A 452 cross react with bovine cells This antibody recognize the intracytoplasmic portion of human CD3, and is recommended for histopathology. I tested the antibody in flow cytometry on bovine PBMC, by intracytoplasmic stain and it works very well. Double staining (surface + cytoplasm) with anti-bovine CD3 showed positivity in CD3 surface positive cells only. IgM positive cells (surface) were negative for cytoplasmic CD3. Hope this help. Cheers, and thanks for the work you're doing. Sometimes is not easy for us (people that don't work with human, mouse o rat) to find the antibody you're dreaming for. Mara Mara Rocchi, DVM Ph.D., Lab 4, International Livestock Research Institute P.O. box 30709 Nairobi, Kenya Fax: 00254-2-631499 Mail: M.Rocchi@cgnet.com
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:09 EST