I want to forward the following question: Does anybody know a good green fluorescent control substance, which does not enter the cell nor bind to the membran of cells. The user want to take it as a control for testing electroporation conditions by subsequent analysis with flow cytometry. It should enter the cell only after opening the membran by electroporation. The substance should be not too big, because it serves as a control for a molecule with molecular weight about 1000. Thanks Andreas ------------------------------------------ Andreas Simm Physiologische Chemie II Biozentrum, Am Hubland D-97074 Wuerzburg FAX: +49-931-888-4113 Tel: +49-931-888-4128 E-Mail: Simm@biozentrum.uni-wuerzburg.de ----------------------------------------
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