Hello everybody,
Has anybody a reliable method to separate monocytes from mouse blood ?
I had problems due to the following :
1) Mouse monocytes density is higher than human monocytes one, so murine
monocytes pass through the usual density gradients (Ficoll-Hypaque,
d=1,077) together with the erythrocytes.
2) Mouse monocytes adhere to plastic or glass when plated but they
completely loose adherence very fast.
Thank you very much,
Victor Turcanu
Department of Immunopharmacology
University Louis Pasteur
BP 24, 74 route du Rhin
67401 Illkirch
France
Tel +33 388 67 69 26
Fax +33 388 66 01 90
e-mail : turcanu@pharma.u-strasbg.fr
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