staining fixed cells -Reply

From: Antony Bakke (bakkea@ohsu.edu)
Date: Mon Sep 15 1997 - 12:12:22 EST


Jan,

Preservation of epitopes after paraformaldehyde depends entirely on the epitope.
 Some are preserved and some are not.  Usually epitopes that are present in high
number are preserved - at least enough to measure.  Other epitopes are not
preserved.  You would have to test each one comparing fixed and unfixed to be
certain.

Happy flowing,
Tony Bakke



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