intracellular staining of proteins in the ER

From: SANDRA NUTI (nuti@iris02.biocine.it)
Date: Mon Sep 15 1997 - 08:46:19 EST

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Hello!
we are interested in the intracellular detection of proteins which are localized
in the endoplasmic reticulum. Has anybody used protocols for permeabilization of
the ER membranes which may be useful to us? Using saponin or saponin and
methanol we have not got reproducible results.
Thank you for your help.
SANDRA NUTI and GIULIANO BENSI
nuti@iris02.biocine.it

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