<<How well does formaldehyde fixation preserve epitopes? In other words, will it be possible to do extensive phenotyping (± 10 CD's) on fixed cells?>> In my experience it depends entirely on what CD's you're looking at. CD2 and CD22, for example, are very sensitive to fixation in formaldehyde/paraformaldehyde and can disappear completely, whereas CD3 is pretty robust. I did have a list of sensitivities of "standard" CD's that I produced a few years ago - can't seem to lay my hands on it though. Of course, it also depends on your fixative concentrations and timings. Wal Sharp SQU Oman
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