Dear group, Our FACS 440 will not display dots or accumulating histograms on the data display screen although pulses appear on the console pulse display (the alpha numerics do appear, however, on the console data display). We started swapping cards and boards from a used 440 we bought for this purpose and replacement of the DMG board in the ND624 restored the dots - for a time. The problem surfaced again the following day! One other piece of information - our software runs on a VAX and on the VAX monitor one can see the dots. We could perhaps operate in this fashion except for the fact that it makes alignment almost impossible, i.e., it's difficult to see the dots representing polystyrene beads on this screen, and they don't seem to respond as quickly (no matter how you set it up timewise) to adjustments in the stream or fluorescence pickup lens as did the dots on the FACS console data display. Does this give anyone a clue as to what has gone wrong? Thanks again for any help. Ray ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Raymond B. Hester, Ph.D. Someday, after we have mastered the Flow Cytometry Lab winds, the waves, the tides and CSAB 357, Biotechnical Services gravity, we shall harness the The University of South Alabama energies of love. Then, for the Mobile, AL 36688-0002 second time in the history of Email: rhester@jaguar1.usouthal.edu the world, man will have Voice: (334) 460-6029 discovered fire. FAX: (334) 460-6073 -Teilhard de Chardin ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
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