Dear Tulips, I am interested in finding a standard method or protocol in the handling of unfixed HIV. Does anyone else sort live HIV+ cells? What safety precautions do you take. The machine is a FACSCalibur, and is in a PC3 containment lab. I understand this is an enclosed flow cell and system, with very little chance of aerosol formation, but? I would be glad if someone gave some comments for comparison. Fanx in advance Geza Paukovics - Flow Laboratory - AIDS Pathogenesis Research Unit Macfarlane Burnet Centre for Medical Research .***. .***. .** PO Box 254 _--_|\ * | | | * * | | Fairfield, VIC 3078 / \ * * | | | * * | | | AUSTRALIA. \_.--._/ * * | | | * | | | * ph. (+61 3) 9282 2132, O '***' '***' FAX (+61 3) 9282 2100
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