DiOC5 from Molecular Probes is a dye that could be considerated in distinguishing dead cells from vital ones ; it measures the membrane potential and enters only into dead cells without damage for the vital ones. We use this dye since 1993 to assess the resistance of microbiological strain to antibiotic therapy by flow Regards Edoardo Colombo MD Director Flow Lab Dept. of Pathology Valduce Hospital COMO , Italy coledoco@tin.it ---------- From: G R Barclay <Robin_Barclay@compuserve.com> To: cyto-inbox Subject: Red cell labels Date: giovedì 11 settembre 1997 17.08 Dear colleagues I am looking for advice on labels which might be used to distinguish damaged and/or dead red cells from healthy ones. Ideally, such a label would readily enter healthy cells, not be quenched by haemoglobin, and be lost from membrane-compromised cells - and would be excitable at 488nm. Alternatively - it might enter cells, *not* be readily lost - and be quenched by haemoglobin - - so that loss of haemoglobin might allow it to emit. Any suggestions?? I may also be looking for similar live/dead markers for platelets - no haemoglobin, but any suggestions and pitfalls? Robin Barclay Scottish National Blood Transfusion Service Edinburgh ----------
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