Bonjour le monde.. I have a friend who wants to stain NK cells for perforin in the secretory granules in combination with 2 cell surface markers . Does any of you have already a good protocol for this, or should we just try conventional intracellular staining procedures ? I would be interested in which antibody clones to use (If there are more than the one from Kamiya), fixation types (PFA, EtOH etc), and other details or tricks in the protocol. Thanks very much for all the hints. Matthias ____________________________________________________________________ Matthias Haury Flowcytometry Dept Immunology Institut Pasteur mhaury@pasteur.fr Tel: 33 (01) 40 61 31 29 Fax: 33 (01) 45 68 86 39 ____________________________________________________________________
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:06 EST