Dear colleagues, We could use some help with a question regarding the sheath system of a Coulter Elite ESP flow cytometer (250 um cuvette with 70 um orifice). We struggle with instability in the fluid/sample system now and then. A simple way of measuring the internal resistance of the system to assess possible fouling or clogging would come handy. Does someone know 1) the typical sheath throughput [ml/min or so] as a function of the pressure P [psi] of the sheath fluid; 2) the typical sample throughput [ul/min or so] as a function of the pressure difference (Psa - Psh) [psi]? Many thanks, George Dubelaar DRIE Bodegraven NL dubelaar@pi.net
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