Dear Flow Chemists: I have been attempting to biotinylate a protein which must be maintained at low pH (around pH3) to retain binding and bio-activity. The protein denatured in 3M guanidine at neutral pH biotinylates well but the activity and binding are killed upon refolding. Can anyone suggest either a biotinylation method or another fluorochrome conjugation that can be done at low pH with a relatively low probability of killing my precious protein ? Steve Neben Genetics Institute
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