Regarding the issue of running radioactive cells through the flow cytometer I have the following comment. We have done this with cells containing low amounts of tritiated thymidine labelled cells. We were quite unsure about it, but decided to try it. We took the cells and measured the cpm, ran them through the cytometer, and then flushed extensively, collecting all the sheath fluid and measuring successive vials collected. We essentially found that there was no activiny after just a very short time - the activity was of course in the cells and once the cells were gone, the instrument was clear - at least we were unable to measure any activity in the wasted sheath fluid after a few minutes. We did subsequent checks and found no activity and concluded that the proceedure was a reasonable one to perform. The instrument was the Elite. Hope this helps. Paul Robinson >I have a group who wish to do flow cytometric analysis (using a Facscan) on >cells that have also been treated with an iodinated ligand. The activity is >"low". I am at the moment rather doubtful about agreeing but I would >appreciate any comments and advice. > > >Many Thanks >Pete > > J.Paul Robinson, Ph.D. Professor of Immunopharmacology Director, Purdue University Cytometry Laboratories EMAIL: robinson@flowcyt.cyto.purdue.edu WEB http://www.cyto.purdue.edu Phone: (765) 494 0757 FAX (765) 494 0517
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:05 EST