On Tue, 02 Sep 1997 13:13:59 -0400 (EDT) KDOMENICO@magnum.mco.edu wrote: > > Hello everyone! I would like to ask those of you who sort on microscope slides > for histochemical stains or FISH for some technical help. We have tried sorting > ficolled cells onto slides using blood bank saline for the sheath fluid. The > sorting goes well but when the slides air dry atfer a sort, the cells lose > their integrity. Is there something else we should be doing? I have been trying > to monitor the bulletin board for other questions on this subject but have not > seen any as of yet. Thank you for your help. > > Karen Domenico (a.k.a. The CCS Volunteer) > Medical College of Ohio > Toledo, Ohio > > Use 1/10 saline as your sheath fluid. This will avoid problems caused by salt crystals as the sheath fluid evaporates. I would also recommend using coated slides (I use polylysine but the SectionLock slides from Polysciences might be worth trying). Michael Ormerod preferred email address: Michael_Ormerod@compuserve.com
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