DNA analysis buffers

From: Mark A. Miller (mamiller@biochem.dental.upenn.edu)
Date: Wed Sep 03 1997 - 08:21:18 EST

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I have a few questions about buffers used in DNA content experiments.

1)  Why is spermine included in the Vindelov/detergent-based systems?
2)  Why do some people use detergents and others use ethanol
permeabilization?
3)  How does one make a buffer for eluting low MW DNA out of apoptotic
cells?

Thanks a lot.  I love this group!

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