You can sort of do this, but you need to be aware that you are measuring the amount of fluorescence from the label, not necessarily the actual receptor. Also you need to know how much fluorescence you are getting from your label. We have come close to an answer by using an avidin-biotin linkage, with a known number of fluorescein molecules on the biotin and using calibration particles with known numbers of fluorescein molecules. Our probe is not an antibody. I would not presume to say that our numbers are actual, just relative. Deb Berglund Montana State University Microbiology On Thu, 28 Aug 1997, Gilbert A. Radcliff wrote: > > Dear Group: > Could someone briefly explain a method by which one can determine > cell surface receptor number in a population of cells? > > I assume that the intensity of staining is proportional to the > density of receptors. > Thank you for your replies! > > Richard Liu, Ph.D. > > Associate Scientist > BMT Group > Moffitt Cancer Center > Tampa, FL > 33612 >
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