I have dabbled in the world of microdrop encapsulation. I can't tell you much about One Cell Systems though, since I didn't use their equipment. My experience was through graduate research. We didn't have quite the budget to lease their microdrop making apparatus. I designed my own apparatus with a threaded plunger syringe, stir plate, lab jack, and ring stand. This technique is outlined in: Gel Microdrop Encapsulation for the Frugal Investigator. In: In Living Color: Flow Cytometry and Cell Sorting Protocols. Edited by Rochelle Diamond and Susan DeMaggio. Landes Bioscience Publishers, Georgetown, TX. Some related articles are: Kenney JS, Gray F, Ancel M-H, Dunne JF: Production of monoclonal antibodies using a secretion capture report web. Bio/Technology 13:787-790, 1995. Powell KT, Weaver JC: Gel microdroplets and flow cytometry: Rapid determination of antibody secretion by individual cells within a cell population. Bio/Technology 8:333-337, 1990. Gray F, Kenney JS, Dunne JF: Secretion capture and report web: Use of affinity derivatized agarose microdroplets for the selection of hybridoma cells. Journal of Immunological Methods. 182:155-163, 1995. Weaver JC, Bliss JG, Harrison GI, Powell KT, Williams GB: Microdrop technology: A general method for separating cells by function and composition. Methods: A Companion to Methods in Enzymology 2:3:234-247, 1991. An alternative to the microdrop encapsulation technique was reported by Rudolf Manz, et al. I know of at least one lab on this forum that was using this technique. (Although I don't remember specifically whose lab). This technique involves direct biotinylation of the cell surface. The reference is: Manz R, Assenmacher M, Pfluger E, Miltenyi S, Radbruch A: Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix. Proc. Natl. Acad. Sci. 92:1921-1925, 1995. Happy Hunting! David McFarland Vanderbilt Medical Center Flow Cytometry Facility _______________________________________________________________________________ Subject: One Cell Systems WWW Page ? + Microdrop Secretion Assays... From: Matthias Haury <mhaury@pasteur.fr> at +inet Date: 08/25/1997 9:08 PM Hello again, I have a couple of questions from a friend concerning the CellSys Agarose Microdrop maker, sold by One Cell Systems, Cambridge MA. 1. Does anybody have the WWW adress, email, tel-fax no etc of "One Cell Systems"? 2. Are there people out there who have used cytokine secretion capture with agarose microdrops with good results ? The idea is to capture the secreted cytokines in the agarose and detect them with flow cytometry. Preferably in living cells, no fixation... cells must be sorted on cytokine secretion and then be still clonable... Any hints, references etc would be appreciated as well. Thanx a lot in advance. Matthias _____________________________________________________________________________ Matthias Haury Flowcytometry Dept Immunology Institut Pasteur mhaury@pasteur.fr Tel: 33 (01) 40 61 31 29 Fax: 33 (01) 45 68 86 39 _____________________________________________________________________________
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:03 EST