On Wed 13 Aug, R Barclay wrote: > > Can anyone suggest reliable sources of antibodies for studies of these FcR > markers on human leucocytes? > Also - has anyone any experience of looking at these in a whole blood (lyse > and wash) method - and are there any pitfalls to be avoided? What blocking > agents are commonly employed (rather not use gammaglobulin)? > Any advice or dialogue welcome. > Robin Barclay > SNBTS, Edinburgh > > Medarex supply antibodies to all three receptors and if you use antibodies which are outside of the IgG binding site you shouldn't have a problem with competition from plasma IgG. Mike Clark, <URL:http://www.path.cam.ac.uk/~mrc7/> -- o/ \\ // || ,_ o M.R. Clark, PhD. Division of Immunology <\__,\\ // __o || / /\, Cambridge University, Dept. Pathology "> || _`\<,_ // \\ \> | Tennis Court Rd., Cambridge CB2 1QP ` || (_)/ (_) // \\ \_ Tel.+44 1223 333705 Fax.+44 1223 333875
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