Lymphocyte Populations

From: Cheryl M. A. Palmer (cpalmer@uwimona.edu.jm)
Date: Thu Aug 21 1997 - 10:31:08 EST 

I use a  FACScan along with Simulset software to enumerate T- lymphocyte
subsets on HIV positive patients. On a few occasions I have seen two
distinct lymphocyte populations. On gating these populations, whether
individually or together, the T+B+NK cells are much lesser than the
ideal 100%.
Can someone give me some ideas as to why this is happening and how I can
 account for the 'missing' cells.

Thank you .
Cheryl Palmer

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