My fresh batch of Quantum 24 beads have 6,000, 16,000, 37,000 and 90,000 MESF molecules respectively in the particle mixture. The fluorochrome is FITC. When I look at them on the fluorescent microscope, I can only see the ones with 90,000 MESF. Is this normal? ....If not, what is the limit for a dark-accomodated eye? Yeah, allow for normal biological variation, by assuming I have 20+/20+ vision! ....If not, what do I do about it? (I know fading is a problem, and have had useful suggestions on this previously. We want to use a high sensitivity, 5 mLux, camera to quantify fluorescence) Thanks, Bob
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