I have a new client that is interested in doing cell-cycle analysis on a neuroblastoma cell line at progressive levels of differentiation. I gave her a general PI staining protocol to start with, but was hoping to get some info more directly related. She's doing a literature search but I thought someone on the list might have some pertinent advice as well. Any hints would be greatly appreciated. David McFarland Vanderbilt Medical Center Flow Cytometry Facility
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