RNA amounts by flow

From: Hoffmann, H. J. (hjh@post1.tele.dk)
Date: Tue Aug 19 1997 - 15:10:24 EST

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I would like to determine the amount of RNA in eosinophils by flow
cytometry to get an idea of the amount of RNA I can expect to extract
from the cells afterwards.  

Does anyone have experience in using LDS 751 or a similar dye to
determine the content of RNA in cells.

All inspiring comments are welcome
Hans Juergen Hoffmann, Ph.D. - Clinical Scientist
       Department of Respiratory Diseases
           Aarhus University Hospital 
                    Denmark
     Tel +45 89 49 21 07 Fax +45 89 49 21 10

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