In response to Dr Millers question on flow viability, Light scatter properties are not a good denominator for either total cell count or viability. Inclusion of a vital dye e.g. 7-AAD will allow detection of dead cells but will not help with the debris problem. If the cells are CD45 positive it would make sense to gate on CD45 positive events and then look at 7-AAD staining only on those events. Feel free to contact me directly if you wish to explore this further. Mike Keeney London Health Science Centre Ontario Canada Mike.Keeney@LHSC.on.ca
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