Re: doxorubicin and viability dyes

From: Mario Roederer (Roederer@Darwin.Stanford.EDU)
Date: Thu Aug 14 1997 - 12:24:47 EST

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>   Hopefully, without giving the impression of being too
>   picky, I need a  viability dye that will:
>   (a) not overlap with doxorubicin's endogenous red
>   fluorescence
>   (b) not employ the mechanism of DNA intercalation so it
>   doesn't        interfere with the cell damaging action
>   of doxorubicin

How about using PE-Annexin V, and then you can use FDA (fluorescein di-acetate)
as a viability dye (it stains LIVE cells rather than dead cells)?  I believe
that PharMingen now has PE-Annexin V; FDA can be purchased cheaply from a number
of sources.

mr

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