B cell precursors

From: Walter Sharp (denby@compuserve.com)
Date: Mon Aug 11 1997 - 22:58:50 EST


A question to the those on the list with experience in early B cells in
normal bone marrow:-

Would you expect SURFACE CD22 to appear before CD20 in normal B cell
development ?

The reason I ask is we had a BM specimen in the other day in which we found
a population of CD19+/10+/20+/22neg/45dim cells which is a phenotype we
have seen on some of our NHL's with marrow involvement (& of course 22 is
neg on most of our CLL's).
I ran out of specimen before I could do any more CD's.
The percentage of these cells is too low to give a definitive diagnosis and
I don't want to mix up lymphoma cells with normal precursors.
Sorry for such a basic question but I deal almost exclusively with ALL's
where CD22 almost invariably appears before CD20 on the surface - at least
with our leuks, which tend to be different from "western" types
We have asked them (another hospital) to send us a suspension from a node
biopsy.


Thanks

Wal Sharp
SQU
Oman (for the time being!)



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