Alice Given writes: [Hello Flowers, Has anyone tried to look at pH measurements on GFP stained cell? We are concerned about the cross-over of GFP into the Snarf channel -- and wonder if there are any other pH probes out there that would be at longer wavelengths. The problem is particularly acute since we are wanting to do this on an imaging system and compensation may be trickier because intensities will vary pixel by pixel. Any ideas?] Dicyanohydroquinone, originally described by Gunther Valet in 1981, is available as an esterified probe from Boeringer-Mannheim. This is a UV excited ratiometric pHi probe that should work well with an imaging system, and avoid fluorescence cross talk with GFP. References can be found by searching on Gunther, Mike Fox, and in a review that Liz Musgrove and I published in Cytometry in the mid 1980's. David Hedley Ontario Cancer Institute/Princess Margaret Hospital Toronto.
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