Hello Flowers, Has anyone tried to look at pH measurements on GFP stained cell? We are concerned about the cross-over of GFP into the Snarf channel -- and wonder if there are any other pH probes out there that would be at longer wavelengths. The problem is particularly acute since we are wanting to do this on an imaging system and compensation may be trickier because intensities will vary pixel by pixel. Any ideas? Alice Alice L. Givan Englert Cell Analysis Laboratory Dartmouth Medical School Lebanon, New Hampshire NH 03756 USA tel 603-650-7661 fax 603-650-6130 e-mail givan@dartmouth.edu
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