One word: karyotype Flow jocks often need to be reminded to use a _microscope_. Seriously, other mouse cells have been shown to produce tetraploid populations. Nocodozole, for one, will even generate octoploidy. Read Cross, Sanchez, Morgan, et al, Science Vol 267, 3 Mar, 1995, pp1353-1356 (Flow AND microscopy!) I don't have the reference of the observation that Canine and human cells DON'T show this type of aneuploidy effect. At 03:31 PM 8/7/97 -0400, you wrote: > >Hi, > > I have an investigator who is analyzing murine embryonic fibroblasts >(MEF) for cell cycle perturbations based on retrovirally infected cell cycle >inhibitory proteins. We have regularly observed what appears to be a true >tetraplod population, ie., exhibiting singlet cell properties based on pulse >processing (FACScan FL-2A vs. FL-2W), PI nucelear isolation prep. We see >this in both normal control and experimental preps. > Is a tetraploid condition a known feature of MEF biology? These are >isolated from fetuses and placed directly into primary culture. We have >seen it often enough that I want to believe it, but it is controversial. >Thanks for the input....regards, Dave G. >Dave Gebhard >Director FACS/ Oligonucelotide Synthesis Facilities >Albert Einstein College of Medicine >1300 Morris Park Avenue >Bronx, New York 10461 >718-430-2724/ 3573 > > > Dennis ************************************************************************* * Dennis J. Young Voice : (619) 822-0407 * * Flow Cytometry Core Facility FAX : (619) 822-0412 * * University of California, San Diego USA e-mail: djyoung@ucsd.edu * * http://www-core.ucsd.edu/flow-core.html * *************************************************************************
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