Can anyone on the list recommend a source of microbeads suitable for daily alignment checks on an instrument used for single channel ploidy/spf analysis via DAPI fluor? We now align using Coulter DNA Check beads, but acquired in another channel since their signal in the DAPI channel is so weak. The multispectral beads from FCSC are a better fit wrt signal strength in the DAPI channel, but they give up a point or two in CV and are more than twice as expensive. We can stay with the Coulter beads, but I'm still searching for a standard that looks as much like the specimen as possible. Thanks for any suggestions. Robert L. Becker, Jr. Col, USAF, MC Department of Cellular Pathology Armed Forces Institute of Pathology Washington, DC 20306-6000 202-782-1573
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