Hi Flowers, We received a specimen of CML for flow and HLA-phenotyping. The peripheral blood count was 280,000 WBC/mm3. The flow went fine but the traditional HLA determination for class I was not feasible because of the high leukemic cells in circulation. As B-cells have also class i receptor on their surface, I decided to go ahead and try performing class I on separated magnetically separated B-cells (traditional technique of class II determination) and the method worked. I would to know if there are other techniques (besides PCR-phenotyping) to go around this kind of frequently encountered problem. Thanks Christian
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