> I would like to get a feeling for the effect of BD's Turbosort option on >the viability of different cell types: human, mouse, rat; PBL's, LN, >thymocytes,splenocytes, bone marrow etc.; mature/immature, healthy or >compromised etc; hybridomas, transfectants etc. Any and all info would be >welcome. Thanks, > >Claude I can't say about the Turbosort since we chose to buy Cytomation MoFlo. However, we have been running regularly at 65psi (that's with a 70um tip and 100K+ drops/sec and 12-20Kcells/sec and 2 lasers). Everything we have put through has had excellent viability. The cells range from thymic and splenic (mouse) lymphocytes,cytokine expanded human dendritic cells, to a wide array of in vitro cell lines. Some of the experiments have used the sorted cells for RT-PCR with excellent results. Many of the experiments involved single cell cloning with excellent cloning efficiency. Part of the success may be due to Cytomation's specially desighned tips. Larry Arnold Univ. of North Carolina
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