Dear all, I need your comments to settle a discussion in the lab regarding PI-based gating of viable cells in FL3. I do membrane staining with a combination of FITC and PE conjugated Moabs, then I stain cels with PI, make exclusion gate on PI-positive cells in FL3 and then evaluate FL1/FL2 staing on PI-negative cells. Is that a correct approach and if not what are other ways? Thank you, Karolina Palucka
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