> Is there anyone with informations about CD34s separation by flow for >transplantation? > >Lucia Silla >Laboratório de Hematologia Celular >Faculdade de Medicina >Pontificia Universidade Catolica >Porto Alegre - RS. BRAZIL Dear Dr. Silla, Most CD34+ cell selection for clinical transplantation is being done by means other than flow cytometry -- avidin-biotin affinity chromatography with anti-CD34 antibody (CellPro's Ceprate column), or immunomagnetic methods (magnetic beads coated with anti-CD34 antibodies, such as Baxter's Isolex instrument, or Amgen's AmCell device). The only company performing CD34 selection by flow cytometry for clinical transplantation, to my knowledge, is Systemix, which uses a high-speed flow cytometer. They can be found on the web at http://www.stem.com/. Other than commercial operations, there are some trials underway at university medical centers involving flow-sorted CD34+ cells for transplant. The CD34+ cells typically are selected by a method other than flow, and then flow cytometry is used to select a CD34+ subpopulation. Here at University of Minnesota, for example, we are involved in a trial of CD34+, HLA-DR- cell transplantation. The CD34+ cells are selected using CellPro's Ceprate device, and then are further purified to isolate CD34+, HLA-DR- cells. This population undergoes retroviral marking and expansion in culture before infusion into the patient. The principal investigator for this protocol is Catherine Verfaillie, and processing is performed in the Cell Therapy Laboratory. Hope this helps you. Scott *************************************************************** Scott R. Burger, M.D. Medical Director, Cell Therapy Laboratory Department of Laboratory Medicine and Pathology University of Minnesota burge009@gold.tc.umn.edu 612-626-4919 612-624-5411 (Fax)
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