It's been awhile since I've last sorted e. coli, but I do remember they didn't like the PBS. Use a different sheath fluid, one provided by your client. I'm pretty sure my client even added ascorbic acid as an anti-oxidant. We also used compressed Nitrogen instead of the house air. Dennis ************************************************************************* * Dennis J. Young Voice : (619) 822-0407 * * Flow Cytometry Core Facility FAX : (619) 822-0412 * * University of California, San Diego USA e-mail: djyoung@ucsd.edu * * http://www-core.ucsd.edu/flow-core.html * *************************************************************************
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