Cell Culture after DNA Ploidy Staining

From: Leonie Gaudry (l.gaudry@unsw.EDU.AU)
Date: Tue Jul 15 1997 - 19:47:57 EST


Hello,
I would like to ask if anyone has a method for estimating DNA Ploidy using
488nm or 633nm Laser so that the cells are alive and sortable before
culturing.If you use Hoechst and UV the above can be done,I am told.My
problem is no UV capabilities hence I am looking for some other way.
Hope someone out there can help me.
Thank you,Leonie



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