There are several references where trypan blue was used to quench the extracellular fluorescence of bioparticles labelled with FITC thereby distinguishing them from intracellular particles resulting from phagocytosis. I would like to do some two color work to look at phagocytosis (of FITC bioparticles) in subsets of mononuclear cells. These would be identified by mAbs and PE. Is this feasible or will the trypan blue also quench PE? Paul Sopp Institute for Animal Health, Compton, UK paul.sopp@bbsrc.ac.uk
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