We have the same experience as Tony Bakke. We are very careful when
preparing cell suspensions and rarely have any clumping unless the tissue
is necrotic. We get the tissue in 30 minutes or less from the O.R. and
gently dissociate the cells into RPMI by repeatedly slicing the lymph node
with a scalpel. Any non-Hodgkin's lymphoma will pour out into the media
with out further manipulations. Hodgkin's disease is another story but then
we don't flow those. If you want our procedure for cell suspension
preparation let me know.
Maryalice
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH
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