I have been working with human PBMC and trying to separate populations. I've found that the B cell markers CD19 and 20 are useful but that they appear to be down regulated after incubation of the cells for 24 or 48 hours, so I can no longer distinguish them as a separate population. Could anybody please suggest a marker that is more stable, preferably one that might be generally available, conjugated to FITC? I'm not a B cell expert as you might gather. I find that the markers CD19 and 20 aren't very bright- much less than CD3 or CD14 for example- is this due to low antigen density, or do I have duff antibodies? Help appreciated. Cheerio, William ______________________________________________ Dr William Smith Institute for Child Health Research (Company Limited by Guarantee ACN 009 278 755) Subiaco, Western Australia, 6008 PO Box 855 West Perth WA 6872 Ph 61 8 9340 8792/8388, Fax 61 8 9388 3414 email williams@ichr.uwa.edu.au ______________________________________________
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