RE: PI staining and washing

From: TIM KUTE (tkute@bgsm.edu)
Date: Wed Jul 09 1997 - 16:12:06 EST


To all:
        Just as a comment about PI staining.  It is not necessary to wash
out the excess stain of PI.   It is known that PI is not very fluorescent
unless it is bound to DNA.   Therefore, the unbound PI will not be measured
by flow cytometry.

Dr. Tim Kute             email:   tkute@bgsm.edu
Associate Professor in Pathology  Tel#: 910-716-2950
Wake Forest Univ-Bowman Gray School of Medicine        Fax#:910-716-7595
Medical Center Blvd
Winston-Salem, NC 27157-1072



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