To all: Just as a comment about PI staining. It is not necessary to wash out the excess stain of PI. It is known that PI is not very fluorescent unless it is bound to DNA. Therefore, the unbound PI will not be measured by flow cytometry. Dr. Tim Kute email: tkute@bgsm.edu Associate Professor in Pathology Tel#: 910-716-2950 Wake Forest Univ-Bowman Gray School of Medicine Fax#:910-716-7595 Medical Center Blvd Winston-Salem, NC 27157-1072
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