Dear colleagues, does anybody know a source for fluorescent beads with changing fluorescence properties (such as excitation of emission spectrum) with the pH value. I want to measure the pH value of the lysosomes of phagocyting macrophages. The beads should have about 2 um diameter. I have beads which show variations, but they are really not monodispers, the fluorescence distribution is extremely broad. I would like to have a sharper distribution. All laser wavelength from UV to HeNe could be used. Thank you in advance Best regards to all flow people Wolfgang Beisker Dr. Wolfgang Beisker GSF - Durchflusszytometrie phone: (+49)(0)89 3187 2842 Ingolstaedter Landstr. 1 FAX : (+49)(0)89 3187 3349 85764 Neuherberg EMAIL: Beisker@gsf.de
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