Dear collegues: In my laboratory work, I attempt to double stain the cells with Brdu (for proliferation assay) and a cell surface marker. The cells I am working with are the cell mixture of intestine epithelial cells and T cells. I don't know if anybody has tried to do the similar things. It seems a little hard to do since the anti-Brdu Ab has to enter the nucleus while the other Ab has to bind to the cell surface moleculels. I would appreciate any input. My email address is wangx01@doc.mssm.edu Tian Wang Clinical Immunology Mt. Sinai School of Medicine Tel:(212)-824-7466
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